Synthesis and angiogenetic activity in the chick chorioallantoic membrane model of thymosin beta-15.

Thymosin beta-15 (Tbeta15), a 44 amino acid peptide (MW = 5173) localized in human prostate and breast cancer tissues was successfully synthesized in multigram quantities using Fmoc solid-phase peptide synthesis. The synthesized product was shown to have the right structure by ESI and MALDI mass spectral techniques and amino acid analysis. Relatively high yield was achieved, which might be due to enhanced acid stability of the p-cyanotrityl resin used. The effect of the synthesized Tbeta15 on the angiogenesis process was investigated using the chick chorioallantoic membrane (CAM) in vivo model. At concentrations above 1 microg/10 microl per disc, Tbeta15 exhibited a positive effect on angiogenesis, comparable to the effect of the intense angiogenetic factor phorbol 12-myristate 13-acetate at a standard concentration of 0.1 microg/10 microl per disc. The results of this study contribute to the further elucidation of the biological regulatory role of thymosin peptides and provide helpful information in the investigation of their possible therapeutic potential. Copyright 2002 Elsevier Science Inc.

胸腺素β-15的合成及其在鸡胚绒毛尿囊膜模型中的血管生成活性。

胸腺素β-15(tbeta 15)，一种位于人前列腺癌和乳腺癌组织中的44个氨基酸的肽(MW = 5173)，使用Fmoc固相肽合成法以数克量成功合成。通过ESI和MALDI质谱技术和氨基酸分析表明合成的产物具有正确的结构。获得了相对高的产率，这可能是由于所用的对氰基三苯甲基树脂的酸稳定性增强。使用鸡胚绒毛尿囊膜(CAM)体内模型研究了合成的Tbeta15对血管生成过程的影响。浓度高于每片1微克/10微升时，Tbeta15显示出对血管生成的积极作用，与标准浓度为每片0.1微克/10微升的强烈血管生成因子佛波醇12-肉豆蔻酸13-乙酸酯的作用相当。这项研究的结果有助于进一步阐明胸腺素肽的生物调节作用，并为研究其可能的治疗潜力提供了有益的信息。版权所有2002爱思唯尔科学公司。