Evidences of protective potentials of microdoses of ultra-high diluted arsenic trioxide in mice receiving repeated injections of arsenic trioxide.

The present study was undertaken to examine if microdoses of ultra-high diluted arsenic trioxide (a potentized homeopathic remedy, Arsenicum Album 200C, diluted 10(-400) times) have hepatoprotective potentials in mice subjected to repeated injections of arsenic trioxide. Arsenic intoxicated mice were divided into: (i) those receiving Arsenicum Album-200C daily, (ii) those receiving the same dose of diluted succussed alcohol (Alc 200C) and (iii) another group receiving neither drug nor succussed alcohol. Two other control groups were also maintained: one fed normal diet only and the other receiving normal diet and Alc-200C. Toxicity biomarkers like aspartate and alanine aminotransferases, glutathione reductase, catalase, succinate dehydrogenase, superoxide dismutase and reduced glutathione contents were periodically assayed keeping the observer “blinded”. Additionally, electron microscopic studies and gelatin zymography for matrix metalloproteinases of liver tissues were made at day 90 and 120. Blood glucose, hemoglobin, estradiol and testosterone contents were also studied. Compared to controls, Arsenicum Album-200C fed mice showed positive modulations of all parameters studied, thereby providing evidence of protective potentials of the homeopathic drug against chronic arsenic poisoning.

超高浓度稀释三氧化二砷对重复注射三氧化二砷小鼠保护作用的证据。

本研究旨在检测超高剂量稀释的三氧化二砷(一种潜在的顺势疗法药物，砷剂Album 200C，稀释10(-400)倍)对反复注射三氧化二砷的小鼠是否具有保肝作用。将砷中毒小鼠分为:(I)每天接受砷剂Album-200C的小鼠，(ii)接受相同剂量的稀释酒精(Alc 200C)的小鼠，和(iii)另一组既不接受药物也不接受酒精。还保留了另外两个对照组:一个只喂正常饮食，另一个接受正常饮食和Alc-200C。定期分析毒性生物标志物，如天冬氨酸和丙氨酸氨基转移酶、谷胱甘肽还原酶、过氧化氢酶、琥珀酸脱氢酶、超氧化物歧化酶和还原型谷胱甘肽含量，使观察者保持“盲态”。此外，在第90天和第120天对肝组织的基质金属蛋白酶进行了电子显微镜研究和明胶酶谱分析。还研究了血糖、血红蛋白、雌二醇和睾酮的含量。与对照组相比，用砷剂Album-200C喂养的小鼠显示出所有研究参数的正调节，从而提供了顺势疗法药物对慢性砷中毒的保护潜力的证据。